Notes on Genus: Caulimovirus

icosahedra

Type member: Cauliflower mosaic virus

Contents

Members of this Genus
General Description
Morphology
Electron Micrograph
Genome
Genus Genomic Organization
Genome Map
Type Member Genomic Organization
All Known Sequences
Curated Sequences

General Description

This is one of 7 genera in the family Caulimoviridae, which are distinctive amongst plant viruses in having double-stranded DNA. Members of the genus Caulimovirus (cauliflower mosaic virus group) have large (45-50 nm diameter) icosahedral particles. Characteristic viroplasms are produced in infected plant cells and are the sites for virus replication and particle assembly. The genus was originally very diverse but only those with 6 or 7 open reading frames are now included in this genus.

Morphology

Virions isometric, not enveloped, 45-50 nm in diameter with no obvious surface structure. The virion shell is multi-layered.

Virions composed of 16 % nucleic acid.

Genome

Monopartite, open circular, double stranded DNA of 7800-8200 base pairs. Genome has discontinuities in both strands: one in the transcribed (alpha- or minus-) strand and one to three in the non-transcribed strand.

Genus Genomic Organization

Seven open reading frames have been identified. Two RNA transcripts are thought to act as mRNAs for translation of the ORFs.

Type Member Genomic Organization

There are believed to be 7 open reading frames:
1 38 kDa Movement protein
2 18 kDa Helper protein involved in aphid transmission
3 15 kDa Thought to be a DNA binding protein incorporated into the virion but now known to co-operate with P2 in aphid transmission
4 57 kDa Coat protein (precursor of 42 kDa protein subunit of the virion shell)
5 79 kDa Viral reverse transcriptase
6 58 kDa Inclusion body protein involved in symptom production, host range etc.
7 11 kDa Unknown function