Notes on Genus: Cytorhabdovirus

enveloped bacilliform

Type member: Lettuce necrotic yellows virus

Contents

Members of this Genus
General Description
Morphology
Electron Micrograph
Genome
Genus Genomic Organization
Genome Map
Type Member Genomic Organization
All Known Sequences
Curated Sequences

General Description

This is one of 2 plant virus genera in the family Rhabdoviridae. It was previously known as Plant Rhabdovirus group A. The genus is characterised by its bacilliform, enveloped, particles which contain a monopartite ssRNA genome and which mature in the cytoplasm of the host plant cell. The name derives from the Greek cyto = cell and rhabdos = rod.

Morphology

Virions regular in shape and size to pleomorphic in shape and size (in unfixed preparations), bacilliform (when fixed prior to negative staining), enveloped, 200-350 nm long and 70-95 nm in diameter. There are distinct surface projections (spikes) dispersed evenly over all the surface. Nucleocapsids filamentous (when uncoiled), with obvious regular cross-banding and with a distinct central canal. Symmetry helical with pitch of 4.2-4.7 nm.

Virions composed of 1 % nucleic acid, 70 % protein and 25 % lipid.

Genome

Monopartite, linear, single-stranded, negative sense RNA, 11000-15000 nucleotides long.

Genus Genomic Organization

The genome organisation is typical of the family with the "standard" genes N, P, M, G and L in order on the template strand. Depending on the species, there are one or more additional genes located between P and M.

Type Member Genomic Organization

There are 6 genes which are listed here in their order on the template strand (i.e. from 3' to 5' with respect to the virion strand):

ORF  Gene     ProductFunction
1 N 50.7 kDaCoat protein (nucleocapsid)
2 P32.5 kDaPhosphoprotein
3 4b33.7 kDaunknown function
4 M 19.5 kdaMatrix protein
5 G62.3 kda Glycoprotein
6 L236.6 kDa   Polymerase