Notes on Genus: Ipomovirus

flexuous filaments

Type member: Sweet potato mild mottle virus

Contents

Members of this Genus
General Description
Morphology
Electron Micrograph
Genome
Genus Genomic Organization
Genome Map
Type Member Genomic Organization
All Known Sequences
Curated Sequences

General Description

This is one of the genera in the family Potyviridae. Like the other genera, it is characterised by its flexuous filamentous particles, inclusion bodies in infected plant cells and by having a polyprotein genome strategy. It differs from most of the other genera in having white fly transmission. Like all the genera except the genus Bymovirus, it has a monopartite genome. It's name is derived from Ipomoea, the generic name of sweet potato, the host of the type member, sweet potato mild mottle virus.

Morphology

Virions filamentous, not enveloped, usually flexuous, 750-950 nm long and 12-15 nm in diameter. Symmetry helical with a pitch of 3.4 nm

Virions composed of 5 % nucleic acid and 95 % protein.

Genome

Monopartite, linear, single stranded, positive sense RNA, about 11,000 nucleotides long. The 3' terminus has a poly(A) tract and the 5' terminus has a genome-linked protein (VPg).

Genus Genomic Organization

Genome organisation is similar to that of other members of the family Potyviridae but shows some variation in detail between members. The genome is translated into a single polyprotein, which is subsequently processed into about ten products, with the coat protein at the C-terminus. Some members of this genus lack the HC-Pro cistron. In some of these cases there appears to be two (divergent) copies of the P1 protein in its place, while in the cassava-infecting members, an additional HAM-1 like protein has been identified between the NIb and coat protein regions. As in other members of the family, an additional small ORF (PIPO) is generated by frameshifting within the P3 cistron.

Type Member Genomic Organization

The genome is translated into a single polyprotein of 390 kDa, from which an estimated ten functional products are produced by proteolytic processing:
P1 protein     putative protease    83 kDa
HC-Pro putative helper component protease    51 kDa
P3 proteinunknown function     34 kDa
"6K1"unknown function     5 kDa
CI Cylindrical inclusion putative helicase    71 kDa
"6K2"unknown function     6 kDa
VPgGenome-linked protein     20 kDa
NIa-ProNuclear Inclusion protease     27 kDa
NIbNuclear Inclusion polymerase     57 kDa
CP Coat protein     34 kDa
An additional small ORF (PIPO) is generated by frameshifting within the third cistron (P3).