Notes on Genus: Pecluvirus

rigid rods

Type member: Peanut clump virus

Contents

Members of this Genus
General Description
Morphology
Electron Micrograph
Genome
Genus Genomic Organization
Genome Map
Type Member Genomic Organization
All Known Sequences
Curated Sequences

General Description

Pecluviruses (from peanut clump virus) had originally been classified with furoviruses but have a different genome structure, including a triple-gene-block on RNA2. The vectors are Polymyxa spp., root-infecting parasites, classified in the Plasmodiophorales.

Morphology

Virions rod-shaped, not enveloped. Usually straight with a distinct central canal. Generally of two modal lengths, 250-300 nm and 92-160 nm, (but difficult to estimate because they are fragile) and about 20 nm in diameter. Symmetry helical. Surface capsomere arrangement obvious. Pitch of helix 2.6 nm.

Genome

Linear, single-stranded, positive sense RNA in two segments. The 3'-terminus has a tRNA-like structure.

Genus Genomic Organization

There are several ORFs on each RNA. Products for replication are encoded on RNA1. The coat protein is at the 5'-end of RNA2 and there is a triple gene block at its 3'-end.

Type Member Genomic Organization

RNA1 (c. 5900 nucleotides) produces:
1     131 kDaReplication protein
2 191 kDaPolymerase readthrough of ORF1, including RNA polymerase motif
3 15 kDaunknown function
RNA2 (c. 4500 nucleotides) produces:
4 23 kDaCoat protein
5 39 kDa ORF2, unknown function
6 51 kDa6-8 =Triple gene block probably involved in cell-to-cell movement
7 14 kDa
8 17 kDa