Notes on Genus: Sapelovirus
Type member: Porcine sapelovirus
The genus Sapelovirus
vian and P
ike viruses) is one of the genera in the family Picornaviridae
. Genome organisation is fairly conserved within the family but members of this genus have a 5'-UTR which has no polyC tract, there is a leader protein and the 2A protein is a protease.
Virions isometric (icosahedral), not enveloped 30 nm in diameter, made of 60 units (protomers) each consisting of one molecule of each of three surface coat proteins.
Monopartite positive sense single-stranded RNA 7.5-8.3 kb in length. There is a genome-linked protein (VPg) at the 5'-terminus and a 3'-polyA tail.
Genus Genomic Organization
The RNA encodes a single polyprotein that is subsequently processed into the functional products. The major structural proteins are encoded near the N-terminus.
Type Member Genomic Organization
The genome of about 7,500 nucleotides is translated into a single polyprotein, which is subsequently processed into twelve products:
|L||9.4 kDa||(Leader protein)|
|1A ||5.7 kDa||Coat protein VP4 (internal capsid protein)|
|1B ||26.2 kDa||Coat protein VP2 (surface capsid protein)|
|1C ||25.9 kDa||Coat protein VP3 (surface capsid protein)|
|1D ||31.6 kDa||Coat protein VP1 (surface capsid protein)|
|2A ||26.3 kDa||protease|
|2B ||11.3 kDa||non-structural protein|
|2C ||38.2 kDa||non-structural protein|
|3A ||11.0 kDa||non-structural protein|
|3B ||2.4 kDa||Genome-linked protein (VPg)|
|3C ||20.2 kDa||protease|
|3D ||52.0 kDa||polymerase|