Notes on Genus: Tospovirus

enveloped spheres

Type member: Tomato spotted wilt virus

Contents

Members of this Genus
General Description
Morphology
Electron Micrograph
Genome
Genus Genomic Organization
Genome Map
Type Member Genomic Organization
All Known Sequences
Curated Sequences

General Description

This is the only plant virus genus in the large family Bunyaviridae. Its name is derived from the type member, tomato spotted wilt virus.

Morphology

Virions pleomorphic in shape and size, spheroidal and enveloped, 80-110 nm in diameter. 3 filamentous nucleocapsids per envelope, each 200-3000 nm long (depending on arrangement) and 2-2.5 nm in diameter. Symmetry helical.

Virions composed of 1-2 % nucleic acid, over 50 % protein and 20-30 % lipid.

Genome

Linear, tripartite ssRNA: L (c.8900 nt), M (c.4800 nt) and S (c.2900 nt). Genomic nucleic acid is negative sense (RNA-L), or ambisense (RNA-M and RNA-S). Nucleotide sequences of 3'-termini are complementary to similar regions on the 5' end, thus forming a panhandle.

Genus Genomic Organization

Translation of the RNA is believed to produce a polymerase from RNA-L (complementary sense), while RNA-M and RNA-S each produces one product in the virus sense and one in the complementary sense. The coat protein is the complementary sense product of RNA-S.

Type Member Genomic Organization

Translation of the RNA is believed to produce the following products:
ORF   Segment   complementary   Size Putative function
1 RNA-L complementary    331.5 kDa    RNA polymerase
2RNA-M viral 33.6 kDa    NSM possibly involved in cell-to-cell movement
3RNA-M complementary 127.4 kDa    precursor of glycoproteins G1 and G2
4RNA-S viral 52.4 kDa    NSS, unknown function
5RNA-S complementary 28.8 kDa   N, nucleoprotein (Coat protein)