Sequence of DPV Beet black scorch virus
Beet black scorch virus isolate Xinjiang variant m163, complete genome.
ACC No: JN635328
Dated: 2012-11-22 | Length: 3644 | CRC: -1149682085
ID JN635328; SV 1; linear; genomic RNA; STD; VRL; 3644 BP.
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AC JN635328;
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DT 07-NOV-2011 (Rel. 110, Created)
DT 22-NOV-2012 (Rel. 114, Last updated, Version 2)
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DE Beet black scorch virus isolate Xinjiang variant m163, complete genome.
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KW .
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OS Beet black scorch virus
OC Viruses; ssRNA positive-strand viruses, no DNA stage; Tombusviridae;
OC Necrovirus.
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RN [1]
RP 1-3644
RX PUBMED; 22971822.
RA Xu J., Wang X., Shi L., Zhou Y., Li D., Han C., Zhang Z., Yu J.;
RT "Two distinct sites are essential for virulent infection and support of
RT variant satellite RNA replication in spontaneous beet black scorch virus
RT variants";
RL J. Gen. Virol. 93(PT 12):2718-2728(2012).
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RN [2]
RP 1-3644
RA Xu J., Wang X., Han C., Li D., Yu J.;
RT ;
RL Submitted (01-SEP-2011) to the INSDC.
RL State Key Lab. for Agrobiotech., China Agricultural University, 2
RL YuanMingyuan Western Road, Beijing 100193, China
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FH Key Location/Qualifiers
FH
FT source 1. .3644
FT /organism="Beet black scorch virus"
FT /host="sugar beet"
FT /lab_host="Nicotiana benthamiana"
FT /isolate="Xinjiang variant m163"
FT /mol_type="genomic RNA"
FT /country="China"
FT /collection_date="16-Apr-2009"
FT /PCR_primers="fwd_name: bb-421, fwd_seq:
FT tgtaatacgactcactatagaagaaacctaaccagtttct, rev_name: bb-412,
FT rev_seq: cccgggcacctggaagaccagg"
FT /note="variant obtained after passage propagation on N.
FT benthamiana in greenhouse"
FT /db_xref="taxon:196375"
FT CDS 36. .2210
FT /codon_start=1
FT /transl_except=(pos:645. .647,aa:OTHER)
FT /product="82K protein"
FT /note="RNA polymerase; ORF2; translational readthrough of
FT ORF1"
FT /protein_id="AER58227.1"
FT /translation="MDSIPYVILRILDFIFHSIFFPSLLFIINHNTTILWACACAYGFY
FT RAFRLIFKIKVEVHPATRAVFKDMVTRFQRESMFSPDDEVPEGIPIHEDVDLVSDPTHK
FT DIKRVRASRRVSYAVRVAHVAKSKVGLLANTKANELVYSRLCRDEMVTHGVRPSHIAHA
FT VPLAVAACFIPLDSDFLAASIRNCDEMEERRAVLGPSYGKXGGLLCTSGFTTPTWRGNP
FT EGLLVKRGPPLAKPRKLYRFSGFGTHIRYGVHDHSLGNVRRGLVERLFMVETKDGLAPT
FT PQPTPGVYAKLSRFHDLVSANLTSTTRLTYEQFLGFYSGRKLERYQQAVESLAIRPIGV
FT QDAWLSTFVKAEKLNISAKPDPAPRVIQPRSPRYNVEVGRFLRHAEEHLFDAINRVYGG
FT RTVFKGLNADQAGMEMQAMWQEFDNPVGIGMDASRFDQHVSKEALEFEHKIWLSMYRGA
FT DRKTLSKLLGMQIHNRGLARCPDGEIRYTVEGCRMSGDINTSSGNCYIMCASVHNYCSQ
FT LGVKRFRLANNGDDCMLVVEAKDEARVRQGLIEYYRELGFTMKVEPTVYELEHLEFCQT
FT RPVLVDGAYRMVRNLHQGMSKDLHSLHDLGSRKAAEAWVSAVGSGGRVMNDGVPVLKSF
FT FMQFPLSSGPKTKSDMSVALQEDWKYKFNRTGCFKNLTPTPQSRYSFWRAFGVLPDEQI
FT ALENGFSRLSFDKLDQDTQEEVSLLQFSGA"
FT CDS 36. .647
FT /codon_start=1
FT /product="23K protein"
FT /note="RNA polymerase; ORF1"
FT /protein_id="AER58228.1"
FT /translation="MDSIPYVILRILDFIFHSIFFPSLLFIINHNTTILWACACAYGFY
FT RAFRLIFKIKVEVHPATRAVFKDMVTRFQRESMFSPDDEVPEGIPIHEDVDLVSDPTHK
FT DIKRVRASRRVSYAVRVAHVAKSKVGLLANTKANELVYSRLCRDEMVTHGVRPSHIAHA
FT VPLAVAACFIPLDSDFLAASIRNCDEMEERRAVLGPSYGK"
FT CDS 2228. .2419
FT /codon_start=1
FT /product="7K protein A"
FT /note="ORF3"
FT /protein_id="AER58229.1"
FT /translation="MEQQRSEQRRERRVRSRSEDRKSMSDVGQSAVNREADVKKDMGPS
FT VSMTVVGENVEFTQHFHF"
FT CDS 2421. .2618
FT /codon_start=1
FT /product="7K protein B"
FT /note="ORF4"
FT /protein_id="AER58230.1"
FT /translation="MSIIYVVQEKPSGFLVWALVVAIVCIIGLLSYTPPERLNHSYHEN
FT NQKTQYITIGGTSTSKVSTN"
FT CDS 2434. .2577
FT /codon_start=1
FT /product="5'K protein"
FT /note="ORF5"
FT /protein_id="AER58231.1"
FT /translation="MSYRRSPLGFSCGHWLLQLCALLDSYRTLHLKDLTTLITKTIRRR
FT NT"
FT CDS 2647. .3345
FT /codon_start=1
FT /product="coat protein"
FT /note="virus shell; ORF6"
FT /protein_id="AER58232.1"
FT /translation="MAPKRNKGGKKSRMSDETVRAPTAGGVIQRTPGIPPRIRSTTIGT
FT RVTNTELLAGVNVAAAGAFSVVGAGLFPSNLGWLNGIASNYSKFRWLAIKLIYIPIVPT
FT TTAGAMTMALSYDPADATPTSFQQVQQMYNSITAPVWAGFDGATVQLLGERPTTGAVCI
FT DVDVNRFGFTWYRYATLAAITALTANDRNLYIPSVCNVATSGGTAATNVGNLMIKYSIE
FT LIEPIPAAIN"
XX
SQ Sequence 3644 BP; 899 A; 867 C; 943 G; 935 T; 0 other;
jn635328 Length: 3644 22-NOV-2012 Type: N Check: 9844 ..
1 aagaaaccta accagtttct cgttgatcag cgatcatgga ttcaatcccg
51 tatgtgatcc tgcgcatact cgattttatc ttccactcca tcttctttcc
101 atctttactc tttatcatca accacaacac caccatcctg tgggcatgtg
151 cctgtgccta tgggttctac cgtgcattcc gcctcatctt caagataaag
201 gtggaagtac acccagccac ccgagccgtc ttcaaggata tggtcactcg
251 cttccagcgg gagagtatgt tctcacctga cgacgaagtg ccggagggca
301 tccctatcca cgaggatgtt gaccttgtta gcgaccccac acacaaagat
351 attaagcggg tccgtgctag caggcgagtc tcttatgccg tgagggttgc
401 ccatgtagcc aagtctaagg tgggattgct cgccaatacc aaggcgaatg
451 agctggtgta ctcccgtctt tgccgagacg agatggttac ccacggtgtg
501 cgcccatcgc acattgcaca cgcagtgccg cttgctgtcg cggcctgctt
551 cataccgttg gacagtgatt tccttgcagc ttctattaga aactgcgatg
601 agatggagga gcggagggcc gtactagggc cctcatatgg aaaataggga
651 ggcctactct gcaccagcgg gtttaccacg cctacttggc gtggtaatcc
701 agagggtttg ctggtgaaga gaggaccacc tctggctaaa cctaggaaac
751 tgtaccgttt ttctgggttt gggactcata tacggtacgg agtgcacgat
801 cactcattgg gcaatgttcg gaggggactt gttgagcggc tattcatggt
851 tgaaaccaag gatggcttag ctccaacacc acagcccacc cctggcgtgt
901 acgccaagtt atcccggttc catgaccttg ttagtgccaa cctaacctcg
951 accaccagat taacatacga gcaattcctc ggattttatt ctggtcgcaa
1001 attagagagg taccaacagg ccgtggagtc gttagcaatc cgcccaatag
1051 gggtacagga tgcttggctt agcacgtttg ttaaggctga aaaattaaac
1101 atctcagcca aacccgaccc ggcacctagg gttattcagc ctaggtcgcc
1151 gaggtacaat gtggaagttg gacggttcct cagacacgct gaggaacatc
1201 tgttcgacgc catcaaccgt gtgtatggtg ggcgaacggt attcaagggg
1251 ttgaatgccg atcaggctgg catggagatg caagctatgt ggcaagaatt
1301 cgacaatcct gtgggtattg gtatggatgc ctctcgtttc gaccaacacg
1351 tctctaagga ggcgttggag tttgaacaca aaatctggct atccatgtac
1401 cgtggggctg acaggaaaac attgtcgaag ctgttgggga tgcaaatcca
1451 caaccgcggt cttgccagat gccctgatgg agaaatcagg tacacggttg
1501 aggggtgtcg tatgtctggg gacatcaaca catcttctgg aaactgctat
1551 atcatgtgtg cttcggtgca caattattgc agccagttgg gtgtcaagag
1601 attcaggctt gccaacaatg gtgatgattg catgcttgtc gtcgaagcca
1651 aggatgaagc acgtgtcagg cagggactca tcgagtatta tagggaattg
1701 ggtttcacca tgaaagtgga acctacagtc tatgaactcg agcacttgga
1751 gttttgccag acacgtccag tccttgtcga tggggcatat cgaatggtgc
1801 gcaatcttca ccagggcatg tctaaggatc ttcactcctt gcacgatctt
1851 ggtagtagga aagctgctga agcttgggtt tcagcggttg gtagtggagg
1901 ccgtgtgatg aatgatggag taccagtgct caaatcattc ttcatgcagt
1951 ttcctctatc ctctggacct aaaaccaagt ccgacatgag tgtagcgttg
2001 caggaagatt ggaaatacaa attcaatcgg actgggtgtt tcaagaactt
2051 gacacccact ccacaatccc gctactcatt ttggcgtgca tttggagtgc
2101 taccagatga acagattgcc ctggagaatg ggttttctcg tctcagcttt
2151 gacaagctgg accaggatac ccaggaggag gttagcctcc tccagttctc
2201 tggggcatga aaacctaacc acttttcatg gaacaacagc gtagtgaaca
2251 acgtcgtgag cgtagagtga gaagtagatc ggaggacagg aagtctatgt
2301 ctgatgtagg gcaatctgct gtcaataggg aagcagatgt caagaaagat
2351 atgggtccat cggtttctat gacggtggtg ggggagaacg tagagtttac
2401 acaacatttc cacttctgaa atgagcatca tttatgtcgt acaggagaag
2451 ccctctgggt ttctcgtgtg ggcattggtt gttgcaattg tgtgcattat
2501 tggactctta tcgtacactc cacctgaaag acttaaccac tcttatcacg
2551 aaaacaatca gaagacgcaa tacataacta ttggaggaac atccactagt
2601 aaagtttcca cgaactgaat tttctgcttt ctagtatagt caataaatgg
2651 cacctaagcg caataaagga ggcaagaagt cccgcatgtc cgatgagaca
2701 gtgcgggctc ctactgcagg gggcgttata caacgcacac ctggcattcc
2751 tccccgcatt aggtccacca ctattggtac gcgtgtcact aacactgagt
2801 tgctcgccgg agtgaatgtc gctgcggcgg gagctttctc agttgttggt
2851 gctggtcttt tccccagcaa ccttggttgg ctcaatggga ttgcttccaa
2901 ttatagcaaa tttaggtggc ttgctatcaa gctcatctac atccccattg
2951 ttcctaccac gaccgctggg gcaatgacca tggctttatc gtatgatcct
3001 gctgatgcta caccaactag tttccaacag gtacaacaga tgtataatag
3051 catcacagca cctgtctggg ctggatttga tggagctact gttcagctgc
3101 taggggagag accaacaact ggggctgtgt gcattgatgt agatgtgaat
3151 cggtttggat tcacatggta tagatatgct acgcttgctg ccattaccgc
3201 actcactgca aatgatagga atctctatat tcctagtgtc tgcaatgtgg
3251 ctacgtctgg tggcactgca gccaccaatg ttggcaatct gatgataaag
3301 tacagcattg agctcatcga gccaatacct gctgccatta attagatccc
3351 acatcctggt gtggctaatc cagtgaaaaa tattaggaaa tccttgggat
3401 tcacacccca gggaggattg cttgcatagt gcaggtatgt cgagtcacac
3451 tatgaggtat tggtgctgaa tcctgggaaa caggcttgac aggtttgggt
3501 ggttccagac cgatgtatta cccaagatac tcatggtact ctactagaaa
3551 acactatgcg tgcgcacacg gctggctatg tcctatcata gctgggggcc
3601 ccggagtgcg aaaccctctt atatacctgg tcttccaggt gccc