Sequence of DPV Grapevine leafroll-associated virus 2
Grapevine leafroll-associated virus 2 isolate GLV2 1050-02 polyprotein (ORF1a), RNA-dependent RNA polymerase (ORF1b), 6.3 kDa small hydrophobic protein (ORF2), putative heat shock protein 70 (ORF3), 63 kDa putative cell-to-cell movemennt protein (ORF4), minor capsid protein (CPm), major capsid protein (CP), 19 kDa protein (ORF7), and 24 kDa protein (ORF8) genes, complete cds.
ACC No: JX513891
Dated: 2012-10-28 | Length: 16489 | CRC: -658245963
ID JX513891; SV 1; linear; genomic RNA; STD; VRL; 16489 BP.
XX
AC JX513891;
XX
DT 28-OCT-2012 (Rel. 114, Created)
DT 28-OCT-2012 (Rel. 114, Last updated, Version 1)
XX
DE Grapevine leafroll-associated virus 2 isolate GLV2 1050-02 polyprotein
DE (ORF1a), RNA-dependent RNA polymerase (ORF1b), 6.3 kDa small hydrophobic
DE protein (ORF2), putative heat shock protein 70 (ORF3), 63 kDa putative
DE cell-to-cell movemennt protein (ORF4), minor capsid protein (CPm), major
DE capsid protein (CP), 19 kDa protein (ORF7), and 24 kDa protein (ORF8)
DE genes, complete cds.
XX
KW .
XX
OS Grapevine leafroll-associated virus 2
OC Viruses; ssRNA positive-strand viruses, no DNA stage; Closteroviridae;
OC Closterovirus.
XX
RN [1]
RP 1-16489
RA Rott M.E., Belton M., Saeed H.;
RT "Detection of grapevine and tree fruit viruses by next generation
RT sequencing";
RL Unpublished.
XX
RN [2]
RP 1-16489
RA Rott M.E., Belton M.;
RT ;
RL Submitted (21-AUG-2012) to the INSDC.
RL Centre for Plant Health, Canadian Food Inspection Agency, Sidney
RL Laboratory, 8801 East Saanich Rd, North Sanich, British Columbia V8L 1H3,
RL Canada
XX
CC ##Assembly-Data-START##
CC Assembly Method :: CLCBio Genomic Workbench v. 4.5
CC Sequencing Technology :: Illumina
CC ##Assembly-Data-END##
XX
FH Key Location/Qualifiers
FH
FT source 1. .16489
FT /organism="Grapevine leafroll-associated virus 2"
FT /host="Vitis vinifera"
FT /isolate="GLV2 1050-02"
FT /mol_type="genomic RNA"
FT /country="Canada"
FT /collection_date="Sep-2010"
FT /db_xref="taxon:64003"
FT gene 95. .8890
FT /gene="ORF1a"
FT CDS 95. .8890
FT /codon_start=1
FT /gene="ORF1a"
FT /product="polyprotein"
FT /note="contains conserved domains for RNA methytransferase,
FT two papain-like endopeptidases and a viral RNA helicase"
FT /protein_id="AFV34733.1"
FT /translation="MSSLAISALPSAVAQLSFGQPAATVAKSFYMTSLPSLQTCFTQCE
FT FSSLFICLASFQKIKTFLSFLSSVRELALFSELSTIGFCCEFIRLGVGEHPLFFVSFQC
FT GPLSVCLGCVNGIFAVLNISFPFLDVASLLMDVGDVVAPTKVRISKREKKLNKRAFSIA
FT RAAARIRRSKVGNKEVRISHVQPLPAGCLRFSQNKNRSELIIIGRDVGVVARVKTAAPV
FT VRRRGSGGLVPLIPLVAVSPFGVRYRCYAPSCGAIACFKNKLASRVAANDSRAEKLLSA
FT RRRKLQQQNFNSRVAKKARDVTASRVDIAEGEVTPPRHIFFGSFSEPFPIPSSSGMARS
FT VLREEPAIVTCGASAPAIKETPRVSVKKADFSPAAPVPREALTPRVAKRGEAIVISGVV
FT HEVLNKIREGVLRFRVGGDMRFSRFFSSTHGCKIVANVRTNTSVWLNCTKVNGEKFSVA
FT IPCSVDYVAMLRYVSGGKFPLVLMSRECYPDGLCYMAHMRYLCAFFCQPFRESDYPLGS
FT WPSVARLKALVLKRFGDEALNIGVRGYYVSRRAFHCDYDSKYARSLMRLVGHVGGDEVI
FT HSTLASTVVLKPGERARDRPKSISFGSFCCELRYVESVNPDAPRLRSEKVLRKVEPRNG
FT VRTESADVGSNVTRKRDARIDRKFSHLLAGSVNKVKKRIAAGVLRYRVGGDMDFHNSFL
FT TQAGYHLLVRRKTSSSVCVELSTPDGRLLRRDVIPCSRDYAAMLSFAAGGRFPLVLMTR
FT DKYKNGYCYLAHCRYASAFLLKGFHPAVFDIGANPTAAKLRSRMVSVLGDRSLSLNLYG
FT SFTSRGIFHCDYDAAYVKDLRFMSAIVAGKEEMGEVVASDITPAMKQKTIEAVYDRLYG
FT GNDSLLKLSIEKDLIDFKNDVQSLKKDRPVIRVPFYMSEVTQNSLTRFYPQFELKFSHS
FT THSDHPAAAASRLLENETLVRLCGHSVSDIGGCPLFHLNSKTQRRVHVCRPVLDGKDAQ
FT RRVVRELQYSSVRSGDDEKILEGPSNVDVCHYPLGVCEHESSTMMMVQVYDASLYEICS
FT AMIKKKARVTYLTMVTPGEFLDGRECVYMESLDCDIEVDVHADVVMYKFGSSCYSHKLS
FT IIKDIMTTPYLVLGGFLFSVEMYEIRMGVNYFKITKSEVCPNISCSKLLRYRRANSDVV
FT KVKLPRFDKKRRMCLPGYDIIYLDSKFVSRVFDYVVCNCSAVNSKTFEWVWSFIKSSKS
FT RVIISGKIIHKDVNLDLKYVESFAAVMLASGVRSRLASEYLAKNLSHFSGDCSFIEATS
FT FVLREKIRNMTLNFNECLLQLVKRVAFATLDVSFLDLDATMESITDFAECKVAVELEEL
FT GCLRANAENEKIRNLAGDSMAAKLASEIVVDIDAKPVAKHAEHPKSEVSEKKEHAKPGL
FT RGGSKAGVLKEFLGFVLSSASRLFSCVADQQRIKSYVHYLDTAVSFLDQNYENLSFIMR
FT VLSKGYSCMFSYLANRGSLANRVASAVQIVREIAVLCANVSVSKAKVVITFSAAVCAMM
FT FNSCGFSGDGREYKSYMHRYTQVLFDTIFFEDSSYLPIELLSSAICGAIVTLFSSGTSV
FT SLNAFLLQITKSFSLELIVRNVVRVTHSLSTTATDGVMRSVFSQIISHLLVGNTGNLAY
FT QIAFLTGVVPLLVKKCVGLIFSMSEDTYSGFIKHGVSEFSFITNILKYLRGQLVDELKA
FT VVQGVFDSNKHLFRDVTQEAIRSTVMQIPTAVVDGLKCAASKVYSGIVGKKTNVGEEKS
FT RGGGDDYEEYFSCDESETPGLRGGSRSGFSILFFFSRLVRRARILIGRIKHRCFSKIFE
FT SISLKIHELRTFLLGKKRSDVGVYEFLSEDVADALSSINECDEIENLIGDGFRDMADDS
FT PTDTNYFDFSDEFLASVNGLPCAGLRGGGRRVGMLAILNYVYNSLSIVARAASKNSTFT
FT SLIIFSRGLVDRIRNIFSNKESVVELVRKYVKNFLKSRMRGLGLRNEHVVNSFGELVPL
FT LLRNKLSETFIYRAVPMSKWCWEVCLSAERREKFFALVCLVTLGFPYFARLLGGVVCDK
FT LLSSLRRAYDRVIVFLKERCDAAMNGLRCLFHFDGNGDSTSAGLKGGGSQTFLWGLLLR
FT LVRFALSLGYAGCKCVFSHHNLFFLCVSLVEEVNMIAKILGGLSFFSRPISSALAFVLL
FT QPERFVEYSGKLSTTFLFFLKISPRVPLKIKGFFEWVAGNPLLRGVRRFLRLIAVKLNL
FT RRKRGLRTRNGGYPSQRAVPVIPSNRVTNDGCERLSEKMQGVEALRSELRILEELDAAV
FT VEKLNKRRNREADDSEFSRPMHESTHDVTSFCSKANSVGTALERAVLVEDAVKSEKISR
FT MAKDLVRKGTVSDEEITNTLSDDEAIEEVPVSEERDDSPKTVRVSEYLNRLNSSFEFPK
FT PIVVDDNKETGGLTNAVREFYYMQELALFEIHSKLCAYYDQLRIVNFERSMAPCNEDAQ
FT LYVRKNGSTIVQGKDVRSNIKDFHDHDFLFDGKISVNRRRRSGNVLYHDNLSFLASNLF
FT LAGYPFSRSFVFTNSSVDILLYEAPPGGGKTTTLIDSFLKVFKKGEVSTMILTANKSSQ
FT VEILKKVEKDISNIECQKRKDKRTPKKSIYTIDAYLMHHRGCDADILFIDECFMVHAGS
FT VLACIEFTRCHKVMIFGDSRQIHYIERNELDKCLYGDLDRFVDLQCRVYGNVSYRCPWD
FT VCAWLSVVYDNMIATVKGESEGKSSMRINEINSVDDLVPDMGSTYLCMLQSEKLEISKH
FT FIRKGLSKLNVLTVHEAQGETYARVNLVRLKFQEDEPFKSIRHITVALSRHTDSLTYNV
FT LAARRGDATCDAIQKANELVNKFRVFPTSFGGSVINLDVKKDVEDNSRCKASSAPLSVI
FT NDFLSDVNPGTTVIDFGDLSADFSTGPFECGASGIVVRDNISSSNITDHDKQRV"
FT gene 8766. .10268
FT /gene="ORF1b"
FT CDS 8766. .10268
FT /codon_start=1
FT /gene="ORF1b"
FT /product="RNA-dependent RNA polymerase"
FT /protein_id="AFV34734.1"
FT /translation="MISEISLQILARDLSSVALAASSCETTSPPATSLITISSVFSAVR
FT SQAIPRRKPSLQENLYSFEARNYNFSTCDRFTSASMFGEAMAMNCIRRCFDLETFSSLR
FT DDVISITRSGIEQWLEKRTPSQIKALMKDIESPLEIDDEICRFKLMVKRDAKVKLDSSC
FT LTKHSAAQNIMFHRKSINAIFSPIFNEVKNRIMSCLKPNIKFFTEMTNRDFASVVSNML
FT GDDDVYHIGEVDFSKYDKSQDAFVKSFEEVMYKELGVDEELLAIWMCGERLSIANTLDG
FT QLSFTIENQRKSGASNTWIGNSLVTLGILSLYYDVRHFEALYVSGDDSLIFSRNKISNY
FT ADDICADMGFETKFMSPSVPYFCSKFVVMCGHKTFFVPDPYKLFVKLGAVKEDVSMDFL
FT FETFTSFKDLTSDFNDERVIQKLAELVALKYDVQSGNTTLALSVIHCLRSNFLSFSKLY
FT PRVKGWQVFYTSVKKALFKSGCSLYESYVTPFGHAVMVWDDE"
FT gene 10361. .10531
FT /gene="ORF2"
FT CDS 10361. .10531
FT /codon_start=1
FT /gene="ORF2"
FT /product="6.3 kDa small hydrophobic protein"
FT /protein_id="AFV34735.1"
FT /translation="MDQVLQFECLYLLNQAVFAITFVFILLIIRVIKSFRRNVHETPIS
FT AVRGQGFSTVV"
FT gene 10540. .12339
FT /gene="ORF3"
FT CDS 10540. .12339
FT /codon_start=1
FT /gene="ORF3"
FT /product="putative heat shock protein 70"
FT /note="HSP70h"
FT /protein_id="AFV34736.1"
FT /translation="MVVFGLDFGTTFSTVCVYKDGKVYSFKQNNSAYIPTYLYLFSETN
FT HMVFGYEAESLMHNAKVRGSFFRDLKRWVGCDSSNFSDYFDRLKPHYAVRPYKIGTGLN
FT DTVIIGNYGGTVRSEVHLPGLIASFIKAMVSCAENAFSCTCTGVICSVPANYDSVQRNF
FT TDQCVSLSGYQCVYMVNEPSAAALSACNLINKKSANLAVYDFGGGTFDVSIISYRNNTF
FT VVRASGGDLNLGGRDVDRAFLTHLFSLTSLEPDLSLDISNLKESLSKTDAEIVYTLKGS
FT DDKREDVRVNKSILTTVMLPYVNRTLNILEATLKSYAKNMGGLRSVKCDLVLIGGSSYL
FT PGLEDILSKHRRIDRILKVADPRAAVAVGCALYSSCLSGSQGLLLVDCAAHTIAIAGMS
FT CDQIICAPAGAPIPFSGTTPLYLPKANRNSQRQIAVFEGEYVKCPRNRKICGSNIKFLD
FT IGVTSVSHSPITFYLDVAISSVGAISFVVRGPEGKEVSLTGTPAYNFSSVVLGTRSVRE
FT LHISLSNKVHLGLLLHRKADRRLLFSEGEAVRYVETVEVADVLKEFKSYNASSLPPDED
FT VEPLMGKSVQKVLRGGRLEEIPL"
FT gene 12266. .13870
FT /gene="ORF4"
FT CDS 12266. .13870
FT /codon_start=1
FT /gene="ORF4"
FT /product="63 kDa putative cell-to-cell movemennt protein"
FT /note="similar to viral heat shock protein Hsp90"
FT /protein_id="AFV34737.1"
FT /translation="MSNHSWGSLFKKYYGEADWRKYLSKSIVAHTANIKTLPDVQLYGG
FT RTVKKSEFESVTPRSFEQELGLLMLSERDVGWCKMCGITVEEAMYQITNPEAYPITAEK
FT CDPDVKTEGQKYTMEDVMNFMRRSNLDINDKILAEQCWSLSNSCGVLINPDDKERFVAL
FT TFKNNELVNDSSNANIDCRVGDYLVYAMSLFNCRSQKSQAGNISLYEKYCAYIRTYLEN
FT TDLYFTSPDRIPLLTGILYDFCKEYNIFYSTYKRNVDNFRFFLTNYMPLISDIFVFQWV
FT KPAPDVRLLFDISAAELTLEVPTLSLVDSQVVIGHVLRFVESYTADPAIDALEEKLDAI
FT MKKSNPHLSTAQLWVGFFCYYGEFRTAQRRVVQRPGVYKTPNSVGGFEINMKNVEEFFD
FT KIQREVPNVSLRRQFNGARAHEAFKVFKKGNISFKPISRLNIPREFWYLNVDYFRHANR
FT SGLSEEEVLILNNISVDVRKLCAERACSTLPSAKRFSKGHKSSVPSLRQEKNYRDPLIT
FT LRNSLYEFRHGNRDRRV"
FT gene 13833. .14504
FT /gene="CPm"
FT CDS 13833. .14504
FT /codon_start=1
FT /gene="CPm"
FT /product="minor capsid protein"
FT /protein_id="AFV34738.1"
FT /translation="MSSGTGIAIGGSEAIETSGFVLSTRKEAVDKFFNELRNENYSSVD
FT SSRLSDQEVKEVLEKSKESFKSELASTDEHFVYHIIFFLIRCAIISTSDKVKYVGSHKY
FT VVEGKTYTVQDVWVFNMIKGLTKQYKRVNGLRAFCCACEDLYLTVAPAMSERFKTKAIG
FT MKGLPVGKEYLGADFLSGTSRLMSDHDRAVSIVAAKNAVDRSAFTGGERKIVSLYDLGR
FT N"
FT gene 14592. .15188
FT /gene="CP"
FT CDS 14592. .15188
FT /codon_start=1
FT /gene="CP"
FT /product="major capsid protein"
FT /protein_id="AFV34739.1"
FT /translation="MELMSDDNLSGLVITDASSLNGADKKLLSAEVIKMLVQKGAPSEG
FT IEIVFGLLLYALAARTTSPKVQRADSDIIFQNTYGDKTVVVTEGDLKKVLEGCAPLNRF
FT TNKPRTFGRTFTEAYIDFCVAYKHKMPQLNAAANLGIPAEDSYLAADFLGACPKLSELQ
FT QSRKMFASMYALKTEDGVVNTPVSNLRQLGRREVI"
FT gene 15185. .15673
FT /gene="ORF7"
FT CDS 15185. .15673
FT /codon_start=1
FT /gene="ORF7"
FT /product="19 kDa protein"
FT /protein_id="AFV34740.1"
FT /translation="MMEICAQNPEALVLLRTNQNTTLLVVKSDDEVNLPKLLICGYLRV
FT SHRGDVTGCNREDIIKDFEGAHHTVIRSRTVQYDYESAVKEYNNADCVVKFFLETGDVF
FT WFFLQSDIKGRAARHLRTFFEANNFFFGSHCGTMEYCLKQVLIETESVIESFCEERNR"
FT gene 15675. .16292
FT /gene="ORF8"
FT CDS 15675. .16292
FT /codon_start=1
FT /gene="ORF8"
FT /product="24 kDa protein"
FT /protein_id="AFV34741.1"
FT /translation="MRVIVSPYEAEDILTRSTEMLRNIDSGALDTKECIEAFSTLTRDL
FT HCAKASYQWCVDTGLCRRDCAEKRLIDTVESNLRLAQPLIREKVATHFCKDEPKELVAF
FT ITRKYVKLTGVAAREAVKIEMRTLSKTVLNKLSLEMAFYISPRAWKNAEWLDLRYTPLK
FT IYRDLLLDVDTLNALCEEDEVHVVVTNKDEDENHDLELQEDC"
XX
SQ Sequence 16489 BP; 4236 A; 3422 C; 4213 G; 4618 T; 0 other;
jx513891 Length: 16489 28-OCT-2012 Type: N Check: 7333 ..
1 ctttcacctg tgtttgtgcc tcggttcaac cacaacctga aagtttcagt
51 tcccaggagt atcggttttt cctcagaaac cttacttctt cagaatgtct
101 agccttgcta tttctgctct tccctccgcg gttgctcaat tgagttttgg
151 tcagcctgct gccacggttg ccaagtcatt ttacatgact tcccttccgt
201 cccttcagac ttgcttcact cagtgcgagt tttcctctct ttttatttgt
251 ttggcttctt tccaaaaaat aaaaacattt ctttctttcc taagttcagt
301 gcgtgaactc gctctttttt ctgaactgtc cacgattgga ttctgctgtg
351 aattcattcg cttaggtgtt ggagaacatc ctttgttttt tgtttccttc
401 cagtgcggac ctttgtccgt gtgtttgggg tgtgtgaatg gtatttttgc
451 cgttctcaac atttcttttc cctttctcga cgttgcttcg ttgttgatgg
501 acgttggtga tgtggtcgct ccgaccaagg taagaatttc caaacgagag
551 aagaagttga ataagcgcgc tttttcaata gctcgtgctg ctgcgcgtat
601 taggcgttcc aaggttggaa ataaggaggt tcgcatctct catgtgcagc
651 ccctccctgc tggttgctta cggttttccc aaaacaaaaa cagatcagaa
701 ctcatcataa taggtcggga cgtaggtgtt gtggcgcgcg taaaaaccgc
751 agcacctgtt gtgcggcgtc gcggtagcgg cggtcttgtt cccctcatac
801 ccctagtagc cgtttcgcct tttggggtga ggtaccgttg ttacgcgcct
851 tcatgtggcg cgatcgcgtg tttcaagaat aagctcgcta gccgtgttgc
901 cgctaacgat agtcgcgccg agaagcttct ttcagctcgg cgccgtaaat
951 tgcagcaaca aaatttcaac agtcgtgtgg cgaagaaagc gcgtgacgta
1001 actgcgtcgc gcgtcgatat agcagaaggt gaagtcacac caccgcgcca
1051 catctttttt ggtagttttt cggaaccctt tccaatacct tcttcttccg
1101 gaatggcgag atcggtgctt cgtgaagaac ccgctatcgt tacgtgtggc
1151 gctagcgcgc ccgcgataaa agaaactccg cgggtgagtg tgaaaaaggc
1201 ggatttttca ccggcggcgc ctgttccgcg ggaggctctt accccaagag
1251 tcgctaaacg cggtgaagcg atcgtcatat cgggcgtggt gcacgaagtt
1301 ttgaataaga ttagagaggg cgttttgcgc tttcgcgtgg gcggtgatat
1351 gcgtttctct aggttcttct catcaaccca cggttgcaaa attgttgcca
1401 acgtgcgcac caacacttcc gtgtggttga actgcactaa ggtcaatggt
1451 gaaaaatttt cggtggcgat cccttgttcg gtggattatg tagcgatgct
1501 gcgttacgtg agtggcggga agttcccgct tgtcctgatg agcagggaat
1551 gttaccctga tgggctatgt tacatggctc acatgaggta tctgtgtgct
1601 ttcttttgcc aacctttcag agaatcagac tatcctctgg gttcgtggcc
1651 atcggtggcg cgattgaagg cgttggtttt gaagcgcttt ggtgacgagg
1701 cgctgaatat aggcgttcgc ggctactatg tctctcgtag ggcttttcat
1751 tgcgactacg attctaaata cgcgagaagc ttgatgaggc ttgtggggca
1801 tgtcggggga gacgaggtta tacattcaac gttggcgtcc acggtcgtgt
1851 taaaaccggg tgaacgcgca cgcgatcgtc cgaaaagcat aagttttgga
1901 tcgttttgct gcgagttgcg ttacgtcgag tccgtcaatc ctgacgcccc
1951 tcgcttgcga agtgagaagg ttctgaggaa agtggagccg cgcaatgggg
2001 ttcgcacgga aagcgctgac gttggttcga acgtcacacg gaagcgcgac
2051 gcccgcatcg atagaaagtt ttcgcatctt ttagcgggaa gcgttaataa
2101 agtgaagaaa cgaatcgcgg ccggggtgtt gcgatatcga gtcggcggtg
2151 acatggactt tcataactcc tttcttaccc aagcgggcta ccacttgcta
2201 gtgcggcgta agacgagcag cagtgtttgc gtggaacttt cgacgccgga
2251 cggtaggctc ttgcgccgcg acgtcatacc ctgctcaaga gattacgctg
2301 cgatgttgtc gtttgccgca ggaggtcgtt tcccgttggt tttaatgacg
2351 agggataaat acaagaacgg ttattgttac ttggctcact gtaggtacgc
2401 gagcgctttt cttctgaagg gttttcatcc tgcggttttt gacattggcg
2451 ctaacccgac ggcggcgaag cttagatcgc gcatggtttc ggtcctaggg
2501 gacagaagtc tgagtttgaa tttatacggg tctttcacgt cccgcggtat
2551 ttttcactgt gattacgacg cggcttatgt gaaagattta agattcatgt
2601 cagcgatcgt ggctggtaag gaggaaatgg gagaggtggt tgcttctgat
2651 ataactccgg cgatgaaaca gaagactatc gaagctgttt acgataggtt
2701 gtatggtggt aacgattcgt tgttgaaatt gagcatagag aaagatctaa
2751 tagatttcaa gaatgacgtt cagagcctga agaaggatag acccgttatt
2801 agggtgcctt tctacatgtc tgaagtcact cagaactcgc tgacgcgttt
2851 ctatcctcaa ttcgagctta aattctcaca ctctacgcac tctgatcatc
2901 ccgcggcggc cgcttcgagg ctgctagaga acgaaacgtt agtgcgattg
2951 tgcggtcaca gtgtttcgga cattgggggt tgtcctctct tccatcttaa
3001 ctcaaaaacg caaaggcgcg ttcacgtgtg ccgacccgtg ttagacggta
3051 aagacgcgca gcgtcgagtg gtgcgcgaat tgcagtattc ttcagtacga
3101 tcgggagatg atgagaaaat tttggaaggg ccctccaatg tggacgtgtg
3151 tcactaccct ctcggcgttt gtgagcacga gagtagtacc atgatgatgg
3201 tgcaggttta cgatgcatcg ctctatgaga tatgcagtgc tatgattaag
3251 aagaaggcgc gcgtaactta cctcactatg gtcactcctg gcgagtttct
3301 cgacgggcgt gaatgtgttt acatggaatc gctagactgc gatattgagg
3351 ttgatgtgca tgcggatgtt gtgatgtata agttcggtag ttcgtgctat
3401 tcgcataagt tatcgatcat taaagatatt atgaccacgc cgtatcttgt
3451 gctcgggggc tttttattta gtgtggaaat gtacgaaatc cgaatgggcg
3501 tgaactactt caagatcacg aagtcggaag tgtgccccaa tatctcatgt
3551 tctaagctgc tgagataccg cagggctaac agcgacgtcg tgaaagtgaa
3601 actgccgcgt tttgataaga agcgtcgcat gtgtttaccc gggtacgata
3651 ttatatactt agattcaaaa tttgtgagtc gtgttttcga ctacgtagtg
3701 tgtaattgtt ccgcggtgaa ctcaaagact ttcgaatggg tctggagttt
3751 cattaagtcc agcaaatcga gagtgattat tagtgggaag atcattcata
3801 aagatgtcaa ccttgatctc aagtacgtcg agagtttcgc tgccgtgatg
3851 cttgcgtcgg gtgtgcgcag caggttggct tcggagtatc tcgcgaagaa
3901 tctcagtcat ttttcgggag attgctcctt tatcgaagcg acctcttttg
3951 ttctgcgtga gaagattaga aatatgaccc ttaacttcaa tgaatgtctt
4001 ctgcagttgg tgaaacgcgt ggcatttgcg actttagacg taagtttctt
4051 agatctggat gccactatgg aatcaatcac agacttcgcc gaatgcaaag
4101 tagctgtgga acttgaagag ttgggatgtt tgagagcgaa tgccgagaat
4151 gagaaaattc ggaacctcgc tggcgactca atggcagcga aattggctag
4201 cgagatagtg gtcgacatag acgcgaaacc tgtcgctaaa cacgcagaac
4251 acccgaagag tgaagtgagt gaaaagaagg agcacgcgaa acccggcttg
4301 cgtggaggct cgaaagccgg agttttgaaa gagttcctcg gtttcgttct
4351 ttcgtccgcg tcgcgcttgt tcagttgcgt ggcggatcaa caacgaatta
4401 agtcgtacgt ccattacctt gacaccgctg tatcgttctt agatcagaat
4451 tacgaaaacc tctccttcat tatgcgcgtt ctatcgaaag gttactcttg
4501 catgttttca tatctcgcga atcgtggtag tcttgccaat cgcgtggcga
4551 gcgcggtgca aatcgttaga gaaattgctg tgttatgcgc taacgtaagc
4601 gtttccaagg ccaaagtagt gatcactttt tccgcagcgg tgtgcgcgat
4651 gatgttcaac agttgcggct tttcaggcga cggtcgagaa tataaatcat
4701 acatgcaccg ttacacgcaa gtgttgttcg acaccatttt ctttgaagat
4751 agtagctatc ttcccataga gttactcagt tccgcaatat gcggcgctat
4801 cgtcactctt ttctcgtcag gaacttctgt aagtctgaat gctttcctgc
4851 tccaaattac taagagcttc tctttggagc ttatcgtccg gaacgtggtg
4901 cgcgtgacgc attctttgag caccacggcg actgatggag tgatgcgcag
4951 cgtcttctct caaataatca gtcacctgct tgtgggaaac actggtaact
5001 tagcttatca aatagctttt ttaaccgggg tggtgccctt gttagtgaag
5051 aaatgcgtgg gtttgatttt ttcgatgagc gaggacacat actctggctt
5101 tatcaagcat ggggttagtg aattttcgtt catcactaat atcttaaaat
5151 acctgagagg acagctggta gacgaattga aagcggtagt tcaaggagtt
5201 tttgactcga ataaacatct ctttcgtgat gttactcagg aggcgatccg
5251 gtcgaccgtt atgcagatac caaccgccgt ggtggatggt ttgaaatgtg
5301 cggctagtaa agtgtatagc ggaatagtcg gtaaaaagac gaatgttggg
5351 gaagaaaaat cccgtggcgg tggagacgac tacgaagagt atttttcctg
5401 cgacgagagc gaaactccgg gtttaagggg cggatccaga agcggtttct
5451 ccatcctatt tttcttttca aggttagtgc ggcgagcgcg cattttgatt
5501 ggcagaataa agcataggtg cttctcgaaa atttttgaga gtatctcgtt
5551 gaagattcat gagcttagaa cctttctcct tggaaagaag cgaagcgacg
5601 taggggtgta cgaattccta tccgaggacg tagctgatgc tctatcctcg
5651 ataaatgaat gtgacgaaat cgaaaatctg atcggagacg ggtttcgcga
5701 tatggcggat gactctccca cggacacgaa ctacttcgac ttctcagacg
5751 aatttttagc ctccgttaat ggcttacctt gcgctgggtt gcgcggaggc
5801 ggtaggaggg tgggaatgct cgccattctg aattacgttt ataactctct
5851 aagcatcgtg gctagagcag cttcgaaaaa ttcgactttc acatctctaa
5901 tcattttttc gcgcggattg gttgatagaa tccgcaacat tttttcaaat
5951 aaggaaagtg tcgttgagct tgtgcgcaag tacgttaaaa actttttaaa
6001 aagtcgcatg cgcggtttgg gtctaagaaa cgaacatgta gtgaattcgt
6051 tcggggagtt ggtgccactg ctcttacgaa acaagttgtc tgaaactttc
6101 atctatcgtg cggtccccat gtcgaagtgg tgctgggaag tgtgcttgtc
6151 agctgagcgg cgggaaaagt ttttcgcgct cgtgtgctta gtcactctgg
6201 gatttcccta cttcgctcgt cttttgggtg gggtagtgtg cgataagctc
6251 ttgtcttcat tacgacgtgc gtacgaccgt gtgatagttt ttctaaagga
6301 gcgatgtgac gcagccatga atgggttgcg ttgtttattt cattttgacg
6351 ggaatggtga ttccacatct gccggtttaa agggcggtgg ctcgcaaacg
6401 tttctatggg gcttgttatt acgtttagtg cgttttgcgc tatctttggg
6451 ctacgcgggt tgtaagtgcg ttttctcgca ccacaatcta tttttcttat
6501 gcgtctcctt agttgaagag gtgaacatga tcgcgaaaat tctagggggt
6551 ttgtcgttct tctcacgacc aatatcatca gcgctggcat tcgttctttt
6601 acagcccgag aggttcgttg agtactctgg aaagttgagc acgacttttt
6651 tgttttttct caaaatttct cctcgcgtgc ctctcaagat caaagggttt
6701 ttcgagtggg tagccggtaa tcctctattg aggggcgttc ggagattcct
6751 tcgcttgatc gcggtgaagc tgaaccttcg aaggaagaga ggactgagaa
6801 cgagaaatgg tggttacccc agccaaaggg ccgtgccggt tataccatct
6851 aatcgcgtga caaatgacgg atgtgagaga ctgtctgaga agatgcaagg
6901 agtcgaagca ctacgatccg aattaaggat tcttgaagaa cttgatgccg
6951 cagtggtgga gaagctcaac aaacgcagga accgtgaagc ggatgatagc
7001 gagttctcgc gccctatgca cgagagcacg catgatgtga cttccttttg
7051 ttcaaaagcg aattcggtag gcacggccct cgaaagggcg gtgctcgtgg
7101 aagatgcggt gaaatctgaa aagatctcga ggatggctaa agatctggtt
7151 cgtaagggga ctgtttccga tgaggaaatc accaacactt tatctgatga
7201 tgaagcgatt gaagaagttc cagtctccga agagcgcgac gactcaccca
7251 aaacggtaag ggtgagtgaa tatttgaata gattgaactc aagcttcgaa
7301 ttcccgaagc cgatcgtggt ggacgacaat aaggagactg gagggttaac
7351 caacgcggta cgcgaattct actatatgca ggagctcgct ctatttgaaa
7401 tccacagcaa actgtgcgct tactacgatc agttgcggat cgtgaatttc
7451 gaacgctcta tggccccatg taatgaggat gctcagttgt acgttcgtaa
7501 aaacggttcc acaatagtgc agggcaaaga tgtgcgttcg aacatcaagg
7551 attttcacga tcacgatttt ctcttcgatg gcaagatctc cgtaaataga
7601 cgacggcgat ctggaaacgt tctgtaccat gataaccttt cttttctggc
7651 gagcaacctg tttctggcgg gatacccttt ttcacggagt tttgttttca
7701 ctaactcttc ggtggatatt cttctttatg aagctcctcc cggtggtggc
7751 aagaccacga ccctaataga ttccttcctg aaagttttca aaaaggggga
7801 ggtatccacc atgatactaa ccgcgaacaa aagctcgcag gttgagattt
7851 taaagaaggt tgagaaagat atttccaata tcgaatgcca gaaacgcaaa
7901 gataagagaa ctccaaagaa gagcatatac accatcgacg cgtatttgat
7951 gcaccacaga ggttgtgatg ctgacatact ttttatcgat gagtgcttta
8001 tggttcacgc cggtagtgtg ttggcgtgta ttgaattcac taggtgccac
8051 aaagttatga tcttcggaga cagtcgacag attcattata ttgaaagaaa
8101 tgaactcgac aagtgccttt acggagacct tgacaggttc gtagacctgc
8151 aatgtcgtgt ctacggtaac gtgtcgtacc gctgtccatg ggacgtctgc
8201 gcctggttaa gcgttgttta tgacaacatg atcgccaccg ttaagggtga
8251 aagcgaagga aaaagtagca tgcgcattaa cgaaatcaac tcggtggacg
8301 acttagtccc cgacatgggt tctacttact tgtgcatgct tcagtctgag
8351 aagttagaaa tcagtaagca tttcatccgc aaagggctct ctaaacttaa
8401 tgtcttgacc gtgcacgagg ctcaaggtga gacatacgcg cgcgtgaatc
8451 tggtgagatt gaaatttcag gaggacgagc cttttaaatc catcaggcat
8501 ataaccgtcg cattatctag acacaccgac agcttgactt acaacgtcct
8551 agccgctcgg agaggcgacg ccacgtgcga cgctatacaa aaagctaacg
8601 agttggtgaa caagtttcgt gtcttcccga cttctttcgg tggtagcgtt
8651 atcaatctcg acgttaagaa agatgtggag gacaacagta ggtgcaaagc
8701 ttcttcggcg cctttaagtg tgatcaacga cttcttgagc gacgttaatc
8751 ccggcaccac cgtaattgat ttcggagatc tctctgcaga ttttagcacg
8801 ggacctttcg agtgtggcgc tagcggcatc gtcgtgcgag acaacatctc
8851 ctccagcaac atcactgatc acgataagca gcgtgtttag cgcagtgcgg
8901 tcgcaggcga ttccgcgcag aaagccttcc ctgcaagaga atctttattc
8951 ttttgaagcg cggaattaca acttctcgac ttgcgaccgc tttacctcag
9001 cttctatgtt cggtgaagcg atggctatga actgcatacg ccgctgtttt
9051 gatttagaga ccttttcatc tttgcgcgat gatgtgattt ctatcacaag
9101 gtcgggtatt gaacagtggt tggagaagcg tactcccagt caaataaagg
9151 ctttgatgaa agatatcgaa tcaccgctgg aaattgatga tgagatctgc
9201 aggttcaaat tgatggtgaa gcgtgatgct aaggtgaagt tagattcttc
9251 ttgcctaacc aaacacagcg ctgctcagaa cattatgttt catcgtaaaa
9301 gtatcaacgc gattttttca cccattttta acgaagtgaa gaaccgtatc
9351 atgagctgtc ttaagcccaa cataaaattc ttcaccgaga tgactaatag
9401 ggattttgct tccgttgtga gcaacatgct cggtgatgac gatgtttacc
9451 atataggcga agtcgatttc tcgaagtatg acaagtctca ggatgctttc
9501 gtgaaatcct ttgaagaagt catgtacaaa gaactcgggg ttgacgagga
9551 actcttagcg atttggatgt gtggtgaaag gctttccatt gcgaatacgc
9601 ttgacggtca gctgtctttc acgattgaga accagagaaa gtcgggtgcc
9651 tcaaacacgt ggatcggtaa ttctcttgtt acgctgggca tactaagtct
9701 ctattatgac gtgaggcatt ttgaggctct ctacgtctct ggtgacgact
9751 ctctgatttt ttcacgcaac aaaatttcga attacgctga tgatatctgc
9801 gctgatatgg gtttcgaaac gaagtttatg tctccgagtg ttccgtattt
9851 ttgttccaaa ttcgtcgtta tgtgcggaca caagaccttt tttgtgcctg
9901 atccgtataa gttattcgtg aaactgggag ctgtaaagga ggatgtctct
9951 atggattttc tctttgaaac tttcacctcc tttaaggatt tgacttcgga
10001 ttttaacgat gagcgcgtga ttcagaaact cgctgagctt gttgctctca
10051 aatacgacgt tcagagtggt aataccactc tagcgttgag cgtgatacat
10101 tgtttgcgtt ctaactttct atcgttcagc aaactgtacc ctcgtgtgaa
10151 aggatggcag gttttttaca cctccgttaa gaaagcactt tttaagagcg
10201 ggtgctcact ctacgaaagc tacgtcactc cattcggtca tgcagtgatg
10251 gtgtgggatg atgagtagca cgctactctt gcgtagtttt gaacggtact
10301 gtttccgcga ctgtgggatg atcacgtgac atacacgatg tgtgtcactg
10351 tatagatatt atggaccagg ttctgcagtt cgagtgtctg tatctgctca
10401 accaggcggt ttttgcaata acattcgtct tcattctgct catcattcgc
10451 gtgattaaat ctttccgcag aaacgtacac gaaactccaa tatcggccgt
10501 ccgtggtcag ggtttctcga ctgttgtgtg atttgcgaaa tggttgtttt
10551 tggtttagat ttcggtacga ctttttcgac ggtctgcgtg tataaagatg
10601 gtaaggttta ctcattcaaa caaaacaatt ctgcgtacat tccgacatac
10651 ctctaccttt tctccgaaac taatcacatg gtttttggtt atgaagccga
10701 atcactgatg cataatgcga aggtgagagg ctctttcttt agggacttaa
10751 aacgctgggt gggttgcgac tccagcaact ttagtgacta ctttgatcgc
10801 ctgaagccac actatgcggt tcgcccgtat aaaataggta ctggtctgaa
10851 tgatacggtg attataggaa actacggggg taccgttcgt tccgaagtgc
10901 atttgccagg actaatagct tcctttatca aggcgatggt tagttgtgcc
10951 gagaatgcct tttcttgcac ttgcacggga gtaatatgtt cagtgccagc
11001 gaattatgac agtgttcaga ggaatttcac tgatcagtgc gtttccttga
11051 gtggttatca atgtgtgtac atggtcaacg aaccttccgc ggctgcttta
11101 tcagcgtgca atctgataaa caagaagtcg gcgaacttgg ctgtatacga
11151 ttttggaggt gggactttcg acgtgtctat tatttcctac cgcaacaata
11201 cattcgtagt gcgcgcttct ggaggagatt taaatttggg aggaagggat
11251 gttgacagag cctttctgac tcatcttttt tctctaacat ccttagaacc
11301 cgatctttcg ttagacattt caaatctaaa agagtcgttg tctaaaactg
11351 acgcggaaat agtttacacg ctaaaaggga gcgacgataa aagggaggat
11401 gttagagtga ataaaagtat tctcactact gttatgctcc cttacgtcaa
11451 tagaacttta aacattttgg aagcaacact taaatcgtac gcaaaaaata
11501 tgggtgggtt acggagcgtt aaatgcgact tagtgctaat tggtggttct
11551 tcgtatcttc cgggtttaga agatatctta tcgaaacacc gacggatcga
11601 tcgaatattg aaagtcgctg atccccgagc ggcggtcgcg gtcggttgtg
11651 cgctatattc gtcgtgcctt tcaggttcac aagggctgtt attagtcgat
11701 tgcgcggctc acaccatcgc aatagccggt atgagctgtg accagattat
11751 ttgcgctcca gctggagctc ctattccctt ctcggggacc acaccgttgt
11801 acttaccaaa agctaataga aattcacaac gtcaaatcgc agtattcgaa
11851 ggtgaatacg tgaagtgtcc cagaaataga aaaatttgcg gttcaaacat
11901 taaatttctt gatatcggtg tcacaagcgt ttcacactca cccatcactt
11951 tctatttaga cgtagccata tctagtgtgg gggctatctc gtttgtggtg
12001 agaggtcccg aaggaaagga agtttccttg acgggcactc ccgcttacaa
12051 cttttcttct gtagtactcg gaacacgtag tgtccgagag ttgcatatta
12101 gtctaagcaa taaagttcat ctcggcctac ttctacaccg gaaggctgac
12151 cggcggttac tattttccga aggtgaagcg gttcgctacg tggaaacagt
12201 agaagttgcg gatgtgctaa aagaatttaa gagctacaac gctagttctc
12251 taccaccaga cgaagatgtc gaaccactca tggggaagtc tgttcaaaaa
12301 gtactacggg gaggcagatt ggaggaaata cctctctaag agcattgtag
12351 cccacacagc taacataaaa actttgccgg acgttcagtt gtacggcggt
12401 agaactgtga agaagtccga atttgaatcg gtgactccga ggtcgttcga
12451 gcaggaatta ggacttctga tgttaagtga acgggacgtg ggttggtgta
12501 agatgtgcgg gataactgtg gaggaggcaa tgtaccaaat cacgaaccct
12551 gaagcgtatc cgatcacagc ggaaaaatgc gatccagatg tgaaaactga
12601 ggggcagaaa tacaccatgg aagacgtgat gaatttcatg cgtcgttcca
12651 acctagatat aaatgataaa atcttagcgg aacagtgttg gtctctatcc
12701 aattcgtgcg gagttttgat taaccccgat gataaagaac ggtttgttgc
12751 tctcactttt aagaataacg aattggttaa cgattcaagc aacgcgaaca
12801 tagactgtcg cgtgggtgat tacttagttt acgctatgtc cctattcaat
12851 tgtaggagtc agaagtcgca agcgggtaac atttcgctct acgaaaagta
12901 ttgcgcttac atcagaactt acttagaaaa taccgatttg tatttcactt
12951 ctcccgatag gataccgtta ctcaccggca ttctctatga tttctgcaag
13001 gagtataata tcttttattc cacatacaaa cgaaacgttg ataatttcag
13051 gttcttccta acgaattata tgcccttgat ttcagacata ttcgttttcc
13101 agtgggtaaa accggcaccg gacgtgagat tactattcga tataagcgca
13151 gccgaattga ctttagaagt tccaacgtta agcttagtgg attcgcaggt
13201 ggtgatagga catgtcctga gatttgtcga atcatacact gctgatccgg
13251 ctattgacgc gttggaagag aaattggacg ctataatgaa gaagagtaat
13301 cctcatctat ctacggcgca actgtgggta ggattcttct gctattacgg
13351 cgaatttcgc accgcacaac gtcgggtggt gcaaaggccg ggtgtgtata
13401 agacgcccaa ttcggtcgga ggtttcgaga ttaatatgaa aaacgtcgaa
13451 gagttttttg ataagatcca acgagaggtg ccaaacgtgt ctctgcggcg
13501 acaattcaac ggagccagag cgcacgaggc attcaaagta tttaaaaagg
13551 gaaacatcag cttcaaaccc atatctcggt taaacatacc gagggagttc
13601 tggtatctga atgtggatta ttttagacat gcgaacagaa gcggactgag
13651 cgaagaggag gtgctcattc ttaataatat aagcgtagat gttcgtaaat
13701 tgtgtgcgga aagagcgtgc agcactctgc ctagcgcgaa gcgctttagc
13751 aaaggtcata aaagtagtgt gccatcattg cgtcaagaga aaaattatag
13801 agatccgttg atcacgttaa gaaactcttt atatgagttc cggcacggga
13851 atcgcgatag gagggtctga ggccatcgag acctcaggtt tcgtgctatc
13901 tacgcgaaag gaagccgtcg ataagttctt caatgaacta aggaacgaaa
13951 attattcttc ggtcgacagc agtcggttaa gcgaccaaga ggtgaaagaa
14001 gttctggaga aaagtaaaga gagctttaaa agcgaattag cgtccaccga
14051 cgaacacttc gtttatcaca tcatcttctt tctgattcga tgcgccatta
14101 tatccacgag cgataaggta aaatacgtag ggagccataa gtacgtagtc
14151 gaaggaaaga cgtacacagt tcaagacgtt tgggttttta acatgattaa
14201 ggggctgacc aagcagtaca aaagggtgaa tggtttgcgt gctttctgtt
14251 gtgcctgtga agatttgtat ctgacagttg ctcccgcgat gtctgaacgc
14301 ttcaaaacca aggctatcgg tatgaagggc ctacccgtcg gaaaggagta
14351 tttaggtgct gactttctct ctggaacgag tagattgatg agtgatcacg
14401 atagagctgt atccatagtc gcggccaaga acgctgtcga tcgtagcgca
14451 tttaccggtg gagagaggaa gatagttagt ttgtatgact taggaaggaa
14501 ctaagcacgt tgtgctatag tgcgcgtcat aatattaagt actagtgcat
14551 attaacaagc agtagtgttt aaagtgtagt gataagaaac aatggagttg
14601 atgtccgatg ataacttgag cggtctcgtc ataaccgacg cttctagttt
14651 gaatggtgcc gataagaaac tgctgtctgc ggaggtcata aaaatgcttg
14701 tgcagaaggg ggcgcccagc gaaggtattg aaatagtctt tgggctactg
14751 ctctacgctc tcgcagcgag aaccacatcg cccaaagttc agagggctga
14801 ttctgatata atctttcaaa acacttacgg tgacaagact gttgtcgtga
14851 cagaaggtga tctcaagaaa gtgcttgaag ggtgcgcacc tcttaataga
14901 ttcactaaca agccgcgaac tttcggccgc acgtttacgg aggcgtatat
14951 tgatttctgc gtggcttaca agcataagat gcctcagctc aacgcggccg
15001 caaatttggg gataccagca gaagattcgt acctagctgc ggacttttta
15051 ggtgcttgcc cgaaactctc tgaattacaa caaagtagaa aaatgtttgc
15101 aagtatgtac gctttgaaga ctgaggacgg agtagtaaat acgcccgtta
15151 gtaatttgcg ccaattaggt aggagagaag ttatatgatg gaaatttgcg
15201 ctcagaatcc tgaggcacta gtgctattac gcacaaatca aaacacaaca
15251 ttactagtcg tcaagtctga tgatgaagtt aatctgccga aattgttgat
15301 atgcgggtat ttacgcgtgt cgcatcgtgg ggatgtgact ggttgtaatc
15351 gcgaagacat aataaaggat tttgaaggtg cacatcatac cgtcatccgc
15401 tcgagaacgg tacagtatga ttacgagtcc gcagttaaag agtacaataa
15451 cgctgattgc gtggtgaaat tctttttaga aacaggagac gttttttggt
15501 tttttctcca aagtgacatc aaaggaagag cggcgcgaca cttgcgcacc
15551 ttcttcgaag ctaacaattt cttctttgga tcgcattgcg gtaccatgga
15601 gtattgtttg aagcaggtac tgatagaaac tgagtctgta atcgaatctt
15651 tttgtgaaga aagaaatcgt taagatgagg gtaatagtat ctccttatga
15701 agctgaagac attctaacaa gatcaactga aatgttgcga aacatagaca
15751 gtggagcgtt ggatacgaaa gagtgcattg aagcgttctc gacgctaacg
15801 cgagatttgc actgcgcaaa agcatcgtac caatggtgcg ttgacacagg
15851 gttgtgccga cgtgattgcg ccgaaaaacg cttgatcgac accgttgaat
15901 caaatctgag actcgctcaa cctctcatac gcgagaaggt ggcgactcat
15951 ttttgcaaag atgaacccaa agaactagtg gcgttcatca cgcggaaata
16001 cgttaagctc acaggtgtgg cggcgcggga ggctgtaaaa atagaaatgc
16051 gcactctatc taagacagtt ttaaataaac tatctttaga aatggccttc
16101 tatatatcac ctcgagcgtg gaaaaacgca gaatggttag atctcaggta
16151 tacgcctcta aaaatataca gagatctttt actagacgtg gacacactca
16201 acgccttatg cgaggaggat gaagttcacg tcgtcgtgac caataaagat
16251 gaagacgaga atcatgatct cgagcttcaa gaagactgtt aaagtctaaa
16301 attctagaat cgcaatatgt ttgcgagtag taaataaaat cgaacgtggg
16351 tggatctacc tgacgtatca actttagctg ttactgggta attaagctaa
16401 caagtgttag cgtaatgtgt atgttgatga agagaaaata ccgtttctag
16451 atcggtgact tttctcttct tttattttta aaataaaaa