Notes on Genus: Brambyvirus
Type member: Blackberry virus Y
- Members of this Genus
- General Description
- Electron Micrograph
- Genus Genomic Organization
- Genome Map
- Type Member Genomic Organization
- All Known Sequences
- Curated Sequences
General DescriptionThis is one of the genera in the family Potyviridae. Like the other genera, it is characterised by its flexuous filamentous particles, inclusion bodies in infected plant cells and by having a polyprotein genome strategy. It differs from most of the other genera in having a very large P1 protein containing an AlkB domain. The vector is not known. Like all the genera except the genus Bymovirus, it has a monopartite genome. Its name is derived from bramble, one of the common names for the host of the type member, Blackberry virus Y.
MorphologyVirions filamentous, not enveloped, usually flexuous, about 800 nm long and 12-15 nm in diameter. Symmetry helical with a pitch of 3.4 nm
Virions composed of 5 % nucleic acid and 95 % protein.
GenomeMonopartite, linear, single stranded, positive sense RNA, about 11,000 nucleotides long. The 3' terminus has a poly(A) tract and the 5' terminus has a genome-linked protein (VPg).
Genus Genomic OrganizationGenome organisation is similar to that of other members of the family Potyviridae. The genome is translated into a single polyprotein, which is subsequently processed into about ten products, with the coat protein at the C-terminus. An additional small ORF (PIPO) is generated by frameshifting within the third cistron (P3).
Type Member Genomic OrganizationThe genome is translated into a single polyprotein of 394 kDa, from which an estimated ten functional products are produced by proteolytic processing:
|P1 protein|| ||putative protease|| ||84 kDa|
|HC-Pro||putative helper component protease|| ||37 kDa|
|P3 protein||unknown function|| ||41 kDa|
|"6K1"||unknown function|| ||6 kDa|
|CI||Cylindrical inclusion putative helicase|| ||71 kDa|
|"6K2"||unknown function|| ||6 kDa|
|VPg||Genome-linked protein|| ||22 kDa|
|NIa-Pro||Nuclear Inclusion protease|| ||26 kDa|
|NIb||Nuclear Inclusion polymerase|| ||61 kDa|
|CP||Coat protein|| ||41 kDa|