Notes on Genus: Crinivirus
Type member: Lettuce infectious yellows virus
- Members of this Genus
- General Description
- Electron Micrograph
- Genus Genomic Organization
- Genome Map
- Type Member Genomic Organization
- All Known Sequences
- Curated Sequences
General DescriptionThis is one of three genera in the family Closteroviridae, which are characterised by their very long (950-2000 nm) and very flexuous particles. They were previously classified as closteroviruses but are now classified separately because they have a bipartite genome. Infected plant cells contain massive particle aggregates, often organized into banded inclusions. The name is derived from the Latin crinis, = hair.
MorphologyVirions filamentous, not enveloped, very flexuous, 950-2000 nm long and 10-13 nm in diameter. There is a distinct virion tail. Symmetry helical, with pitch of 3.4-3.7 nm.
Virions composed of 5% nucleic acid.
GenomeBipartite, linear, single-stranded, positive sense RNA 15,000 to 20,000 nucleotides long. There are sequences with potential hairpin structure near the 3'-end. The 3' terminus has no poly(A) tract and the 5' terminus probably has a methylated nucleotide cap.
Genus Genomic OrganizationThe genome organisation is broadly similar to the closteroviruses but divided between two RNAs. RNA1 has three or four ORFs, including those involved in virus replication. RNA2 has five or six ORFs, including a heat shock protein homologue and two coat proteins. The ORFs are translated from a set of overlapping, 3'-coterminal subgenomic RNAs.
Type Member Genomic OrganizationThe RNA is translated from eight open reading frames:
|RNA1 has 3 ORFs:|
|1|| 217 kDa||Polyprotein ORF1a, containing a methyltransferase motif|
|2||55 kDa||Polymerase ORF 1b probably expressed by ribosomal frameshift with ORF1a|
|3||32 kDa||unknown function|
|RNA2 has 5 ORFs:|
|4||62 kDa||heat-shock protein analogue|
|5||59 kDa||unknown function|
|6||28 kDa||Coat protein|
|7||52 kDa||Minor coat protein|
|8||26 kDa||unknown function|