Notes on Genus: Pecluvirus
Type member: Peanut clump virus
Pecluviruses (from pe
mp virus) had originally been classified with furoviruses
but have a different genome structure, including a triple-gene-block on RNA2. The vectors are Polymyxa
spp., root-infecting parasites, classified in the Plasmodiophorales
Virions rod-shaped, not enveloped. Usually straight with a distinct central canal. Generally of two modal lengths, 250-300 nm and 92-160 nm, (but difficult to estimate because they are fragile) and about 20 nm in diameter. Symmetry helical. Surface capsomere arrangement obvious. Pitch of helix 2.6 nm.
Linear, single-stranded, positive sense RNA in two segments. The 3'-terminus has a tRNA-like structure.
Genus Genomic Organization
There are several ORFs on each RNA. Products for replication are encoded on RNA1. The coat protein is at the 5'-end of RNA2 and there is a triple gene block at its 3'-end.
Type Member Genomic Organization
|RNA1 (c. 5900 nucleotides) produces:|
|1 ||131 kDa||Replication protein|
|2 || 191 kDa||Polymerase readthrough of ORF1, including RNA polymerase motif|
|3 ||15 kDa||unknown function|
|RNA2 (c. 4500 nucleotides) produces:|
|4 ||23 kDa||Coat protein|
|5 ||39 kDa ||ORF2, unknown function|
|6 ||51 kDa||6-8 =Triple gene block probably involved in cell-to-cell movement|
|7 ||14 kDa|
|8 ||17 kDa|