Notes on Genus: Sapelovirus


Type member: Porcine sapelovirus


General Description

The genus Sapelovirus (from Simian, Avian and Porcine Entero-Like viruses) is one of the genera in the family Picornaviridae. Genome organisation is fairly conserved within the family but members of this genus have a 5'-UTR which has no polyC tract, there is a leader protein and the 2A protein is a protease.


Virions isometric (icosahedral), not enveloped 30 nm in diameter, made of 60 units (protomers) each consisting of one molecule of each of three surface coat proteins.


Monopartite positive sense single-stranded RNA 7.5-8.3 kb in length. There is a genome-linked protein (VPg) at the 5'-terminus and a 3'-polyA tail.

Genus Genomic Organization

The RNA encodes a single polyprotein that is subsequently processed into the functional products. The major structural proteins are encoded near the N-terminus.

Type Member Genomic Organization

The genome of about 7,500 nucleotides is translated into a single polyprotein, which is subsequently processed into twelve products:
L9.4 kDa(Leader protein)
1A  5.7 kDaCoat protein VP4 (internal capsid protein)
1B  26.2 kDaCoat protein VP2 (surface capsid protein)
1C 25.9 kDaCoat protein VP3 (surface capsid protein)
1D 31.6 kDaCoat protein VP1 (surface capsid protein)
2A 26.3 kDaprotease
2B 11.3 kDanon-structural protein
2C 38.2 kDanon-structural protein
3A 11.0 kDanon-structural protein
3B 2.4 kDaGenome-linked protein (VPg)
3C 20.2 kDaprotease
3D 52.0 kDapolymerase