Notes on Genus: Tombusvirus
Type member: Tomato bushy stunt virus
- Members of this Genus
- General Description
- Electron Micrograph
- Genus Genomic Organization
- Genome Map
- Type Member Genomic Organization
- All Known Sequences
- Curated Sequences
General DescriptionThe genus Tombusvirus (tomato bushy stunt virus group) is one of 11 genera in the family Tombusviridae. The genera are morphologically similar, with icosahedral particles and small, (usually) monopartite ss RNA genomes. Members of this genus have larger genomes (>4.6 kb) than other members of the family, 5 open reading frames and do not have the coat protein at the 3'-terminus of the RNA.
MorphologyVirions isometric (icosahedral; T=3), not enveloped and about 30 nm in diameter. The nucleocapsids have an obvious regular, apparently round, surface structure. 32 capsomeres per nucleocapsid (virion composed of 180 protein subunits).
Virions composed of 17 % nucleic acid.
GenomeMonopartite, linear, single stranded, positive sense RNA of length c. 4800 nucleotides. The 3' terminus has no poly(A) tract. The 5' terminus probably has a methylated nucleotide cap.
Genus Genomic OrganizationThe RNA of all members investigated so far is translated into five putative products, including a polymerase, a 40-45 kDa coat protein translated from the larger of two subgenomic RNAs and two other products translated from the smaller subgenomic.
Type Member Genomic OrganizationThe RNA (of the cherry strain) is translated into five putative products:
|2.||92 kDa||Polymerase readthrough|
|3.||41 kDa||Coat protein, translated from subgenomic RNA1 (2156nt)|
|4.||22 kDa||Movement protein, translated from subgenomic RNA2 (935 nt)|
|5.||19 kDa||unknown function, translated from subgenomic RNA2 (935 nt)|