Notes on Genus: Rubulavirus

enveloped spheres

Type member: Mumps virus

General Description

The genus Rubulavirus is one of nine genera in the family Paramyxoviridae and contains viruses that have a hemagglutinin-neuraminidase attachment protein, translate protein V from a non-edited RNA transcript and do not produce a non-strutural protein C. It is named from Rubula inflans, the old name for mumps (caused by the type member).

Morphology

Virions pleomorphic but usually spherical, enveloped, 150 nm in diameter or larger with spike-like projections.

Genome

Monopartite single-stranded negative sense RNA of about 15 kb. There is no genome-linked protein (VPg) at the 5'-terminus nor a 3'-polyA tail.

Genus Genomic Organization

There are 6 or 7 transcriptional elements (mRNA species). Their major ORFs are listed here in their order on the template strand - i.e. from 3' to 5' with respect to the virion strand:

1	N	56-61 kDa	Coat protein (nucleocapsid)
2	P	41-42 kDa	Phosphoprotein produced by RNA editing, creating a frame shift
3	V	24-25 kDa	Produced from the unedited P transcript
4	M	41-42 kDa	Matrix protein
5	F	57-60 kDa	Fusion protein: glycosylated envelope protein
6	SH	5-7 kDa	Small membrane protein (in some members only)
7	H	62-66 kDa	Hemagglutinin-neuraminidase attachment protein
8	L	256-257 kDa	Polymerase

Type Member Genomic Organization

There are 7 transcriptional elements (mRNA species). Their major ORFs are listed here in their order on the template strand - i.e. from 3' to 5' with respect to the virion strand:

1	N	61 kDa	Coat protein (nucleocapsid)
2	P	42 kDa	Phosphoprotein produced by RNA editing, creating a frame shift
3	V	24 kDa	Produced from the unedited P transcript
4	M	42 kDa	Matrix protein
5	F	59 kDa	Fusion protein: glycosylated envelope protein
6	SH	7 kDa	Small membrane protein
7	H	64 kDa	Hemagglutinin-neuraminidase attachment protein
8	L	257 kDa	Polymerase